Free Technology Workshops - 20th March
Free technology workshops (TBC) will be held during the lunch break on 20th March 2013 and are open to everyone, even if you are not attending the main conference.
Please register for the workshop of interest at the time of your registration for the main conference.
However, if you are not attending the main conference but would like attend a technology workshop, please register by sending your name, affiliation and address by email on 'RNAiOxford@gmail.com'.
By registering for a workshop you accept these Terms & conditions.
1.15pm-2.00pm: Technology Worskshop by Sigma Life Science
miRNA tools, esiRNA and Zinc Finger Nucleases : Tools for high-content screens, pathway elucidation, target identification, validation and genome editing
Sigma-Aldrich provides leading functional genomics technologies, which includes RNA interference (RNAi) tools for gene silencing and ZFN's for genome editing. These powerful research tools are used in a variety of applications, including high-throughput screening, the elucidation of pathways, in the identification of new genes and gene function, and in the development of potential therapeutic agents. Sigma’s miRNA, esiRNA (endoribonuclease-prepared siRNA) and ZFN products provide researchers with powerful and specific tools for their experiments. The seminar will discuss in detail Sigma’s miRNA solutions, esiRNA and ZFN technologies, and will highlight each product’s unique features and benefits
2.10pm-2.20pm: Short Technology Worskshop by Carl Zeiss Ltd
Isolating Pure Samples for RNA Analysis with Laser Capture Microdissection (LCM)
LCM is a simple yet powerful microscopy technique, that allows you to:
- Visualise tissue sections or live cell cultures
- Manually or automatically select single cells or areas that you wish to isolate from the remaining sample
- Cut these cells out with a microscopic laser and capture them into a PCR tube (or similar)
The isolated samples are then free to be used in downstream molecular RNA analysis assays. The process of laser capture microscopy is explained, with accompanying examples of how this applies to and is beneficial for RNA investigations. This talk references work carried out for research into cancer, neuroscience, immunology and many other fields.