J Venom Res (2020), Vol 10, in press
Published online: 20 July 2020
Raphael Schezaro-Ramos 1,2, Rita C Collaço 2, José C Cogo 3, Cháriston A Dal-Belo 4, Léa Rodrigues-Simioni 2, Thalita Rocha 5, Priscila Randazzo-Moura 1,2*
1 Laboratory of Pharmacology, Department of Physiological Sciences, Pontifical University Catholic of São Paulo (PUC/SP). Rua Joubert Wey, 290, Vila Boa Vista, 18030-070, Sorocaba, SP, Brazil
2 Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP). Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887, Campinas, SP, Brazil
3 Serpentarium of the Centre for Nature Studies, Vale do Paraíba University (UNIVAP). Avenida Shishima Hifumi, 2911, Urbanova, 12244-000, São José dos Campos, SP, Brazil
4 Federal University of Pampa (UNIPAMPA). Av. Antônio Trilha, 1847, Centro, 97300-162, São Gabriel, RS, Brazil
5 São Francisco University (USF). Avenida São Francisco de Assis, 218, Jardim São José, 12916-900, Bragança Paulista, SP, Brazil
*Correspondence to: Priscila Randazzo de Moura, Email: firstname.lastname@example.org, Tel: +55 15 997154849
Received: 05 May 2020 | Revised: 07 July 2020 | Accepted: 15 July 2020
© Copyright The Author(s). This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction of this article, provided the original work is appropriately acknowledged, with correct citation details.
Philodryas olfersii produces similar local effects to Bothrops jararacussu snakebite, which can induce misidentification and bothropic antivenom administration. Antivenom therapy is effective but has limitations regarding local damage; since plants are used in folk’s medicine to treat snakebites, we evaluated the protective properties of Cordia salicifolia and Lafoensia pacari extracts against Philodryas olfersii and Bothrops jararacussu venoms. Preparations pretreatment with both extracts inhibited > 90% the B. jararacussu venom-induced neuromuscular blockade, and 52% to 81% the P. olfersii venom-induced blockade. C. salicifolia inhibited the myonecrosis promoted by both venoms, however, L. pacari prevented only the myofilaments hypercontraction. Regarding haemorrhagic activity, C. salicifolia was more effective against B. jararacussu venom while L. pacari was more effective against P. olfersii venom. On the other hand, for oedema-forming activity the results were the opposite. Considering that both extracts prevented (to different levels) the main manifestations of both snakebites (local symptoms), we endorse further studies involving these plants as coadjuvant in snakebite therapeutics.
KEYWORDS: Neuromuscular junction, myotoxicity, haemorrhage, oedema, protective effect, antiophidic