In ovo RNAi opens new possibilities for temporal and spatial control of gene silencing during development of the vertebrate nervous system

Review Article

J RNAi Gene Silenc (February 2006), 2(1), 126-135

doi: jrgsxx

Published online: 28 February 2006

Full Text: (html | pdf ~412kb | refs)

In ovo RNAi opens new possibilities for temporal and spatial control of gene silencing during development of the vertebrate nervous system

Thomas Baeriswyl and Esther T Stoeckli *

Institute of Zoology, University of Zurich , Winterthurerstrasse 190 , 8057 Zurich , Switzerland

*Correspondence to: Esther Stoeckli, Email: Esther.Stoeckli@zool.unizh.ch, Tel: +41 44 6354840, Fax: +41 44 6356879

Received: 01 December 2005, Revised: 27 January 2006, Accepted: 03 February 2006

© Copyright The Authors

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ABSTRACT

Loss-of-function approaches are important tools for functional gene analysis. Due to the availability of sophisticated methods to manipulate gene expression in embryonic stem cells that can be used to generate mutant mice, the mouse is by far the most important vertebrate model organism for basic and applied biomedical research. Unfortunately, the available methods do not allow for precise temporal and spatial control of gene silencing during embryonic development limiting the usefulness of the mouse for developmental studies. Due to their easy accessibility chicken embryos have been one of the preferred model organisms for developmental studies. Their disadvantage, the lack of genetic tools, could be overcome by the development of in ovo RNAi (in ovo RNA interference), a method that allows for temporal and spatial control of gene silencing in vivo.

KEYWORDS: RNAi, chicken embryo, in ovo RNAi, in utero RNAi, nervous system, embryonic development

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