Local and systemic biochemical alterations induced by Bothrops atrox snake venom in mice

Research Report

J Venom Res (2013), Vol 4, 1-4

doi:

Published online: 15 March 2013

Full Text: (html | XML | pdf ~170kb)

Analysis of nociceptive effects of neurotoxic phospholipase A2 from Vipera nikolskii venom in mice

Igor A Dyachenko †, Arkadii N Murashev †, Tatyana V Andreeva ‡, Victor I Tsetlin ‡, Yuri N Utkin ‡*

Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow region, Russia

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

*Correspondence to: Yuri Utkin, Email: utkin@mx.ibch.ru, Tel: + 007 495 3366522 , Fax: + 007 495 3366522

Received: 18 November 2012, Revised: 07 March 2013, Accepted: 15 March 2013

© Copyright The Authors

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ABSTRACT

Phospholipases A2 are represented in snake venoms by several types and possess diverse biological activities including neurotoxicity. Earlier we have isolated and characterized two neurotoxic phospholipases A2 (HDP-1 and HDP-2) from the venom of Nikolski’s viper (Vipera nikolskii). They are heterodimers composed of two non-covalently bound subunits. Each heterodimer consists of an enzymatically active basic subunit and an inactive acidic subunit. In this work, we studied the in vivo biological activity of HDP-2 in mice. The acute toxicity (LD50=0.38μg/gm) and maximal tolerated dose (0.1μg/gm) were determined. In the hot plate test HDP-2, at the maximal tolerated dose, reliably prolonged the time of the mouse staying on the plate. However, taking into account the neurotoxicity of HDP-2, we believe that this effect may be explained by a general intoxication rather than specific decrease of pain sensitivity. In this respect HDP-2 differs from other heterodimeric phospholipases A2 like crotoxin which possess analgesic activity. This difference can be explained by a dissimilarity in the structure of the acidic subunits, suggesting an important role of this subunit in analgesic activity.

KEYWORDS: phospholipase A2, toxicity, venom, snake, nociception

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