Purification of a phospholipase A2 from Daboia russelii siamensis venom with anticancer effects

Research Article


J Venom Res 
(2011), Vol 2, 42-51

doi:

Published online: 22 October 2011

Full Text: (html | XML | pdf 1767kb)

Purification of a phospholipase A2 from Daboia russelii siamensis venom with anticancer effects

Suchitra Khunsap †‡, Narumol Pakmanee †, Orawan Khow †, Lawan Chanhome †, Visith Sitprija †, Montamas Suntravat ‡, Sara E Lucena ‡ , John C Perez ‡ , Elda E Sánchez ¥*

† Queen Saovabha Memorial Institute, Bangkok 10330, Thailand

‡ National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, MSC 158, 975 West Avenue B, Kingsville, TX 78363, USA

¥ Department of Chemistry and NNTRC, Texas A&M University-Kingsville, MSC 161, Kingsville, TX 78363, USA

*Correspondence to: Elda Sánchez, E-mail: elda.sanchez@tamuk.edu, Tel: +1 361 593 3796, Fax: +1 361 593 3798

Received: 08 September 2011, Revised: 15 October 2011, Accepted: 17 October 2011

© Copyright The Authors

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ABSTRACT

Venom phospholipases A2 (PLA 2) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA 2 (Drs-PLA 2) was purified from Daboia russelii siamensisvenom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange high performance liquid chromatography (HPLC). The molecular weight of the Drs-PLA 2 was 13,679Da, which was determined by MALDI-TOF mass spectrometry. Its N-terminal amino acid sequence was homologous to basic PLA 2s of viperid snake venoms. The Drs-PLA 2 had indirect hemolytic and anticoagulant activities, cytotoxic activity with a CC 50 of 65.8nM, and inhibited SK-MEL-28 cell migration with an IC 50 of 25.6nM. In addition, the Drs-PLA 2 inhibited the colonization of B16F10 cells in lungs of BALB/c mice by ~65%.

KEYWORDS: Daboia russelii siamensis, p hospholipase A 2, cytotoxic, cell migration inhibition, lung tumor colonization

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