Vector-based siRNA delivery strategies for high-throughput screening of novel target genes

Review Article

J RNAi Gene Silenc (August 2005), 1(1), 5-11

doi: jrgsxx

Published online: 27 July 2005

Full Text: (html | pdf ~308kb | refs)

Vector-based siRNA delivery strategies for high-throughput screening of novel target genes

Meihong Chen †, Quan Du ‡, Hong-Yan Zhang ‡, Claes Wahlestedt ‡§, and Zicai Liang ‡*

† Chinese Human Genome Center North, Beijing, 100176, China and Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology, Beijing, 100005, PR China

‡ Center for Genomics and Bioinformatics, Karolinska Institutet, 17177, Stockholm, Sweden

§ Department of Drug Discovery, Scripps Florida, Jupiter, FL 33458, USA

*Correspondence to: Zicai Liang, Email: Zicai.Liang@cgb.ki.se, Tel: +46 8 5248 6371, Fax: +46 8 323950

Received:10 June 2005, Revised: 16 June 2005, Accepted: 19 June 2005

© Copyright The Authors

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ABSTRACT

Application of siRNA in high-throughput fashion is still in its early phase although the principle has been established for three years. In this review, we outline the different vector-based siRNA delivery platforms as well as resources that are becoming available for high-throughput applications, and some initial outcomes of vector siRNA high-throughput screening efforts using vector encoded siRNA. It is expected that further improvement of the siRNA technology and availability of the siRNA resources will help to materialize the potential of siRNA for functional genomics and drug target validation.

KEYWORDS: siRNA, RNAi, vectors, gene silencing, siRNA delivery

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