Dear Colleagues:
We are delighted to announce our second Oxford symposium on aptamers, Aptamers 2015, which is designed to bring together academic and industrial aptamer researchers and solution providers. The symposium will address therapeutic, diagnostic, analytical as well as basic research applications of aptamers and invites proposals for podium and poster presentation. Please contact us on AptamersOxford@gmail.com, should you require any further information.
Aptamers 2015 will be co-hosted with our 1st symposium on antisense and catalytic oligonucleotides, Oligo 2015, on 30 March 2015.
We cordially invite you to the beautiful and historical city of Oxford to what we hope will be a memorable meeting.
Dr Vittorio de Franciscis
(Symposium Chair)
Research Director
CNR Institute of Experimental Endocrinology and Oncology
Naples
Italy
08.30-09.20: Registration, welcome coffee and networking
09.20: Housekeeping announcements
09.25: Welcome by the symposium chair
09.30: Dr David Bunka
Co-director and CTO of Aptamer Solutions and Aptamer Diagnostics Ltd, UK
Title: Aptamers: Where’ve we been, where are we now and where are we going
10.00: Professor Said Ismail
Head, Molecular Biology Research Laboratory, Medical School, University of Jordan, and, Head, Molecular Diagnostics Lab, Jordan University Hospital, Amman, Jordan
Title: Aptamers as Potential Tyrosine Kinase Inhibitors
10.30: Professor Dr Ulrich Hahn
University of Hamburg, Department Chemistry, Institute for Biochemistry and Molecular Biology, Martin-Luther-King-Platz 6, Hamburg, Germany
Title: Cell surface protein specific aptamers for diagnosis, therapy and cargo delivery into target cells
11.00: Refreshment break, posters, exhibition, networking
11.30: Dr Sarah Shigdar
Lecturer in Medical Sciences, Deakin University, VIC, Australia
Title: Getting the brain to take its medicine
11.50: Professor Yoshikazu Nakamura
President and CEO, Ribomic Inc, Tokyo, Japan
Title: Aptamer therapeutics targeting pain and lung fibrosis
12.10: Dr Georg Mahlknecht
Immunology Department, Weizmann Institute of Science, Israel
Title: Aptamers targeting growth factor receptors
12.30: Dr Paloma H Giangrande
Associate Professor, Department of Internal Medicine, University of Iowa, USA
Title: RNA aptamer smart drug for the treatment of vascular injury
13.00: Lunch and networking
14.00: Professor Nagy Habib
Professor of Hepatobiliary Surgery, Faculty of Medicine, Department of Surgery & Cancer, Imperial College London, UK
Title: Targeted RNA activation
14.30: Dr Vittorio de Franciscis
Research Director, CNR Institute of Experimental Endocrinology and Oncology, Naples, Italy
Title: Combined selective targeting of cancer stem cells by aptamer-miRNA/antimiR conjugates
15.00: Professor Dr Beatrix Suess
Synthetic Genetic Circuits, Technical University of Darmstadt, 03 064 Schnittspahnstraße 1064287, Darmstadt, Germany
Title: Control of alternative splicing by engineered riboswitches
15.30: Refreshment break, posters, exhibition, networking
16.00: Dr Thomas Schubert
2bind GmbH, Regensburg, Bavaria, Germany
Title: Characterisation of aptamer-target interactions using MicroScale Thermophoresis
16.30: Professor Dr Günter Mayer
Life and Medical Sciences (LIMES)- Institute, University of Bonn, Gerhard-Domagk-Str. 1, Bonn, Germany
Title: A modular approach to nucleobase-modified aptames
17.00: Professor Karolien De Wael
AXES Research Group, Department of Chemistry, Antwerp University, Belgium
Title: Electrochemical aptasensing devices
17.20: Dr Arnaud Buhot
University of Grenoble/CNRS/CAE, France
Title: Gold nanoparticles SPRi enhanced signal for small molecules detection with split aptamer
17.40: Dr Julian Tanner
Associate Professor, Department of Biochemistry, University of Hong Kong, China
Title: DNA aptamers for point-of-care malaria diagnosis – from crystal structure to clinical application using Aptamer Tethered Enzyme Capture (APTEC)
18.00: Dr Graeme Doran
Director of Genomic Assays, FireFly BioWorks, Cambridge, MA, USA
Title: Direct quantification of small nucleic acid delivery to tissues
18.30: Close
19.00: NETWORKING DINNER (By invitation or prior booking only)
9.20: Dr Jean-Jacques Toulmé
Director, Institut Européen de Chimie et Biologie, Pessac, France
Title: Aptamer driven supra-molecular assembly
09.50 Dr David Porciani
NEST – Scuola Normale Superiore, Piazza San Silvestro 12 Pisa, Italy
Title: Aptamer-mediated co-delivery of doxorubicin and NF-kB decoy enhances chemosensitivity of pancreatic tumor cells
10.20: Dr Nebojsa Janjic
SomaLogic, Inc, 2945 Wilderness Place, Boulder, CO, USA
Title: Modified aptamers with enhanced chemical diversity as essential tools for biomedical research
10.40: Refreshment break, posters, exhibition, networking
11.10: Professor Weian Zhao
Assistant Professor, Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, CA, USA
Title: Microencapsulated aptamer sensors for digital quantification of blood biomarkers
11.30: Dr Maureen McKeague
Bioengineering Department, Stanford University, USA
Title: Advancing a development pipeline for RNA switches through scalable measurement platform
11.50: Dr Yoann Roupioz
University of Grenoble/CNRS/CAE, France
Title: On the use of aptamer microarrays as a platform for the exploration of human prothombin/thrombin conversion
12.10: Mr Nam Nguyen Quang
CEA, Molecular Imaging Research Center, France
Title: Improvement of aptamers combining doped-SELEX and next-generation sequencing
12.30: Lunch and close
Fully Automated In Vitro Aptamer Selection
Edward Barnes
Aptamer Solutions Ltd, Suite 2.80 – 2.87, Bio Centre, Innovation Way, Heslington, York, YO10 5NY, UK
Semi-automated aptamer selection for biomarker discovery
Edward Barnes
Aptamer Solutions Ltd, Suite 2.80 – 2.87, Bio Centre, Innovation Way, Heslington, York, YO10 5NY, UK
Next-generation analysis of deep sequencing data: Bringing light into the black box of SELEX experiments
M Blank and C Gröber
AptaIT GmbH, Munich, Germany
Identification of tumour specific aptamers by in vivo SELEX
Laia Civit1, Holger Weber2, Michael Kubbutat2, Günter Mayer1
1Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk Str. 1, 53121 Bonn, Germany
2Research Division ProQuinase, Breisacher Str. 117, 79106 Freiburg, Germany
The development of a drug delivery system using brain endothelial-specific non-antibody binding domains as blood brain barrier transport carriers
Mohammad Daas 1, David Male 1, Carol Midgley 1, George Thom 2, Carl Webster 2, Ignacio A Romero 1
1 Department of Life, Health, and Chemical Sciences, The Open University, Walton Hall, Milton Keynes, MK7 6AA, United Kingdom
2 MedImmune, Milstein Building, Granta Park, Cambridge, CB21 6GH, United Kingdom
Selection of LNA-containing DNA aptamers against recombinant CD73
Nicola Derbyshire1, Ida C. Elle1, Kasper K. Karlsen1, Mikkel G. Terp2, Niels Larsen3, Ronni Nielsen4, Susanne Mandrup4, Henrik J. Ditzel2,5, Jesper Wengel1
1 Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
2 Institute of Molecular Medicine, University of Southern Denmark, J. B. Winsløwsvej 25, 5000 Odense C, Denmark
3 Danish Genome Institute, Skt. Lucas Kirkeplads 8, 8000 Aarhus C, Denmark
4 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
5 Department of Oncology, Odense University Hospital, Sdr. Boulevard 29, 5000 Odense C, Denmark
Aptamer Biosensor Based on Fluorescence Resonance Energy Transfer for Multiplexed Pathogenic Bacteria Determination
Nuo Duan, Shijia Wu, Zhouping Wang
State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Aptamers Targeting Dendritic cells and Macrophages
Silvana Hassel1, Verena Schütte2, Susanne Schmidt2, Joachim Schultze2, Sven Burgdorf2 and Günter Mayer1
1Life and Medical Sciences (LIMES), University of Bonn, Gerhard-Domagk-Str. 1, Germany
2Life and Medical Sciences (LIMES), University of Bonn, Carl-Troll-Str. 31, Germany
Phage Display and SELEX brought together – a bivalent aptamer-antibody conjugate for VEGFR specific targeting
Przemyslaw M Jurek, Marta Matusiewicz, Mateusz Piksa, Maciej P. Mazurek, Piotr Jakimowicz, Filip Jelen
Pure Biologics Ltd, Wroclaw, Poland
Selection and Identification of single-stranded DNA Aptamers Targeted to Cronobacter sakazakii
Hong-Seok Kim, Young-Ji Kim, Jung-Whan Chon, Dong-Hyeon Kim, Jin-Hyeok Yim, Dasom Choi, Il-byeong Kang and Kun-Ho Seo
Center for Food Safety, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea
Resampling of aptamer libraries based on aptamer family consensus motifs to isolate the highest affinity binders
Andrew B Kinghorn1, Julian A Tanner1
1Department of Biochemistry, Faculty of Medicine, University of Hong Kong, 21 Sassoon Road, Hong Kong SAR
Aptamer Mediated Uptake of Therapeutic Agents
Sven Kruspe1, Cindy Meyer2, Uli Hahn1
1University of Hamburg, Department Chemistry, Institute for Biochemistry and Molecular Biology, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany
2 Laboratory of RNA Molecular Biology; Howard Hughes Medical Institute; The Rockefeller University; New York, NY USA
The use of a bi-functional aptamer to target brain metastases
Joanna Macdonald, Wei Duan and Sarah Shigdar
School of Medicine, Deakin University, Waurn Ponds, VIC, Australia, 3216
Isolation and characterization of RNA aptamer for P. falciparum cytoadherence
VideoScan AptaBead Array – Identification of optimized buffer/binding conditions for Aptamers using a Multiplex, High-throughput Screening Platform
Carsten Schmidt1, Christian Schröder2, Peter Schierack3, Stefan Rödiger1
1 InnoProfile-Transfer RG “Imagebased-assays”, Brandenburg University of Technology, Großenhainer Str. 57, 01968 Senftenberg, Germany
2 Chair of Molecular Biology, Brandenburg University of Technology, Großenhainer Str. 57, 01968 Senftenberg, Germany
3 Chair of Molecular Diagnostics, Brandenburg University of Technology, Großenhainer Str. 57, 01968 Senftenberg, Germany
Click-SELEX – A modular method enabling nucleobase-modified aptamers
Fabian Tolle and Günter Mayer
Life and Medical Sciences Institute (LIMES), University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany
Detection of Small Molecules using Sandwich-type Enzyme-linked Aptamer Kissing Complex Assay (ELAKCA)
Benoît Chovelon1,2, Guillaume Durand3, Eric Dausse3, Jean-Jacques Toulmé3, Patrice Faure2,4, Eric Peyrin1*, Corinne Ravelet1
1CNRS UMR 5063, Department of Molecular Pharmacochemistry, University Grenoble Alpes, 38400 St Martin d’Hères, France
2 Département de Biochimie, Toxicologie et Pharmacologie, CHU de Grenoble site Nord – Institut de biologie et de pathologie, F-38041 Grenoble, France.
3 ARNA Laboratory, Inserm U869, University of Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France.
4Inserm U1042, Laboratory of hypoxy physiopathology study, University Grenoble Alpes, 38700 La Tronche, France
An Improved Design of the Kissing Complex-based Aptasensor for the Detection of Adenosine
Emma Goux1, Samuele Lisi1, Corinne Ravelet1, Guillaume Durand2, Emmanuelle Fiore1, Eric Dausse2, Jean-Jacques Toulmé2 and Eric Peyrin1
1 CNRS UMR 5063, Department of Molecular Pharmacochemistry, University Grenoble Alpes, 38400 St Martin d’Hères, France
2 ARNA Laboratory, Inserm U869, University of Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France
Selection and application of DNA aptamers against Shigella sonnei
Zhouping Wang, Nuo Duan, Shijia Wu
State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Aptamex kmirage – a bioinformatics application to compare next-gen sequencing SELEX data
Albert J Vilella,Willi Gottstein, Andy Ballard, Kasper Piskorski, Jesper Nissen, Weiyan Xiang, Lucy Colwell, Andrew Deonarine
Aptamex Limited, an Onto.it Software company, 62-66 Victoria Road, Cambridge, CB4 3DU, UK
Macrocyclic host-dye reporter for sensitive sandwich-type fluorescent aptamer sensor
Cheng YANG 1,2, Nicolas SPINELLI 2, Sandrine PERRIER 1, Eric DEFRANCQ 2 and Eric PEYRIN 1
1 Département de Pharmacochimie Moléculaire, Université Grenoble Alpes, CNRS UMR 5063, Grenoble, France
2 Département de Chimie Moléculaire, Université Grenoble Alpes, CNRS UMR5250, Grenoble, France
Gold Sponsor and Exhibitor
Aptamer Solutions Ltd is a York based Biotechnology Company specialising in the custom selection of high-affinity and highly specific nucleic acid aptamers for use in the life sciences sector. Our proprietary automated high-throughput aptamer selection processes allow us to offer a flexible and competitive pricing structure for the development of RNA and DNA aptamers. In addition, we are about to launch a new complementary technology in the area of biomarker discovery.
Our proprietary aptamer based biomarker discovery platform and proprietary combinatorial libraries contain up to and over 1018 different molecules, this diversity and bespoke library design is fundamental to the success of the screening process. This technology enables us to greatly speed up the identification of novel biomarkers as well as diagnostics and/or therapeutic candidate molecules. This technology builds on one of the most powerful uses of aptamer technology, which is the ability for aptamers to be isolated against targets without any prior knowledge of the target.
Our aptamer-based proteomic technology identifies novel biomarkers within the cell surface (such as tumours, cell lines or stem cells) or in samples of biological fluids (such as, urine, plasma and saliva). The technology is versatile and can also be applied to viruses, bacteria, fungi or any other cell based materials or extracts. The discovery process is driven by identifying differences between sample population using enormous aptamer libraries. Samples are prepared based on broad classifications such as: disease vs normal; pre-metastatic vs. post metastatic cancers; pathogenic vs non-pathogenic fungi etc. The process is fast and efficient and identifies differences between ‘healthy’ and ‘diseased’ whilst simultaneously developing the affinity reagent. This cuts out many of the steps associated with traditional biomarker discovery processes.
Silver Sponsor
The 2bind GmbH, Regensburg, Germany, provides biophysical analyses of molecular interactions. We work with worldwide customers from pharma, biotech as well as universities on the characterization of molecular interactions in terms of binding affinities, stoichiometries and binding energetics. Our semi-automated platform based on the MicroScale Thermophoresis technology (MST) allows us to offer cost-efficient, precise and fast analyses of molecular interactions. The integrated quality controls of the MST system enable us to easily identify sticking and aggregation effects and to directly improve measurement conditions ensuring high quality data. In addition to immobilization-free measurements in free solution, MST offers free choice of buffer, allowing close to native measurements in sera and lysates. Besides the low sample consumption our customers benefit from the broad application range of the MST. With our strong expertise in assay development, the company established standardized assays to analyze aptamer-target interactions in semi-high throughput for the aptamer developing industry and scientists. Further MST services includes competition assays, binding site mapping assays and binding assays that simultaneously test multiple binding partners. Hence, our services based on the MST technology are perfectly suitable for the identification, characterization and optimization of aptamer-target interactions.
