OxfordOxford Mon - Fri 09:00-17:00 +44(0)1865-600222
OxfordOxford Mon - Fri 09:00-17:00 +44(0)1865-600222
J Venom Res
Aptamers 2019
03-04 April 2019, Oxford
Submit a manuscript

Isothermal titration calorimetry studies of aptamer-small molecule interactions: practicalities and pitfalls



ISSN: 2514-3247
(2018), Vol 2, in press

Published online: 12 June 2018

Full Text Access 

Sladjana Slavkovic and Philip E. Johnson*

Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, Toronto, Ontario, Canada, M2R 1A1

*Correspondence to: Philip Johnson, Email: pjohnson@yorku.ca, Tel: 1 416 736 2100 x33119

Received: 31 January 2018 | Revised: 09 June 2018 | Accepted: 11 June 2018

© Copyright The Author(s). This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction of this article, provided the original work is appropriately acknowledged, with correct citation details.


Isothermal titration calorimetry (ITC) is a powerful technique for studying binding interactions. From a single ITC experiment it is possible to quantify the affinity and thermodynamics of a binding event. Here, we outline an experimental approach for performing ITC experiments with a focus on aptamer-small molecule interactions. We also discuss some common problems that can be encountered and how to resolve these issues.

KEYWORDS: Aptamers, isothermal titration calorimetry, binding thermodynamics